Thursday, March 26, 2009

The post where you all get to laugh at me

I've been shit with posting the last week or so because I've been busy writing lab reports, assignments, and memorizing the fucking nuclei in the brainstem. So as penance, I'll post this gel from two weeks ago:

I my defense:

  • I wasn't the only one responsible for this mess (four pairs of hands are rarely better than one)
  • It was my first time pouring a gel
  • I hadn't slept the night before.


Anonymous said...

I don't know what it's supposed to look like, so I can't mock you.

DuWayne Brayton said...

I don't know what it's supposed to look like either, but screw it...


That's what you get for lack of sleep and lack of experience.

Though you did a considerably better job than I would have done.

Ambivalent Academic said...

How on earth did you get the ladder to run in two different directions at the same time!?!? The force must be strong in you.

LostMarbles said...


I have no fucking clue, honestly. I've been trying to figure it out for a while now and I keep drawing a blank. When I asked my TA, he mumbled something and looked confused. I know that the two problems in preparation were:
- not enough agarose (We mixed it in glassware that couldn't go in the microwave and didn't have enough time to redo it, so we just poured it into a different flask and lost agarose)
- The two bubbles that formed in the gel

I don't know how this could contribute to the DNA ladder looking like that or if maybe the problem is something else we hadn't considered. This all makes me feel really dumb.

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